Everything about Dna Nanotechnology totally explained
DNA nanotechnology is a subfield of
nanotechnology which seeks to use the unique
molecular recognition properties of
DNA and other
nucleic acids to create novel, controllable structures out of DNA. The DNA is thus used as a structural material rather than as a carrier of biological information, making it an example of
bionanotechnology. This has possible applications in
molecular self-assembly and in
DNA computing.
Introduction: DNA crossover molecules
Structure of the 4-arm junction.
Left: A schematic. Right: A more realistic model.
Each of the four separate DNA single strands are shown in different colors.
DNA nanotechnology makes use of branched DNA structures to create DNA complexes with useful properties.
DNA is normally a linear molecule, in that its axis is unbranched. However, DNA molecules containing junctions can also be made. For example, a four-arm junction can be made using four individual DNA strands which are
complementary to each other in the correct pattern. Due to Watson-Crick
base pairing, only portions of the strands which are complementary to each other will attach to each other to form
duplex DNA. This four-arm junction is an immoble form of a
Holliday junction.
Junctions can be used in more complex molecules. The most important of these is the "double-crossover" or DX motif. Here, two
antiparallel DNA duplexes lie next to each other, and share two junction points where strands cross from one duplex into the other. This molecule has the advantage that the junction points are now constrained to a single orientation as opposed to being flexible as in the four-arm junction. This makes the DX motif suitible as a structural building block for larger DNA complexes.
Tile-based arrays
DX arrays
DX molecules can be equipped with
sticky ends in order to combine them into a two-dimenstional periodic lattice. Each DX molecule has four termini, one at each end of the two
double-helical domains, and these can be equipped with sticky ends that program them to combine into a specific pattern. More than one type of DX can be used which can be made to arrange in rows or any other
tessellated pattern. They thus form extended flat sheets which are essentiallt two-dimensional
crystals of DNA.
DNA nanotubes
In addition to flat sheets, DX arrays have been made to form hollow tubes of 4-20
nm diameter. These DNA nanotubes are somewhat similar in size and shape to
carbon nanotubes, but the
carbon nanotubes are stronger and better conductors, whereas the DNA nanotubes are more easily modified and connected to other structures.
Other tile arrays
Two-dimensional arrays have been made out of other motifs as well, including the
Holliday junction rhombus array as well as various DX-based arrays in the shapes of triangles and hexagons. Another motif, the six-helix bundle, has the ability to form three-dimensional DNA arrays as well.
DNA origami
As an alternative to the tile-based approach, two-dimensional DNA structures can be made from a single, long DNA strand of arbitrary sequence which is folded into the desired shape by using shorter, "staple" strands. This allows the creation of two-dimensional shapes at the
nanoscale using
DNA. Demonstrated designs have included the
smiley face and a coarse
map of
North America. DNA origami was the cover story of
Nature on
March 15,
2006.
DNA polyhedra
A number of three-dimensional DNA molecules have been made which have the connectivity of a
polyhedron such as an octahedron or cube. In other words, the DNA duplexes trace the edges of a polyhedron with a DNA junction at each vertex. The earliest demonstrations of DNA polyhedra involved multiple
ligations and
solid-phase synthesis steps to create
catenated polyhedra. More recently, there have been demonstrations of a DNA
truncated octahedron made from a long single strand designed to fold into the correct conformation, as well as a tetrahedron which can be produced from four DNA strands in a single step.
DNA nanomechanical devices
nanorobotics. One of the first such devices, called "molecular tweezers," changes from an open to a closed state based upon the presence of control strands.
DNA machines have also been made which show a twisting motion. One of these makes use of the transition between the
B-DNA and
Z-DNA forms to respond to a change in buffer conditions. Another relies on the presence of control strands to switch from a paranemic-crossover (PX) conformation to a double-junction (JX2) conformation.
Applications
Algorithmic self-assembly
DNA nanotechnology has been applied to the related field of DNA computing. A DX array has been demonstrated whose assembly encodes an
XOR operation, which allows the DNA array to implement a
cellular automaton which generates a
fractal called the
Sierpinski gasket. This shows that computation can be incorporated into the assembly of DNA arrays, increasing its scope beyond simple periodic arrays.
Note that DNA computing overlaps with, but is distinct from, DNA nanotechnology. The latter uses the specificity of Watson-Crick basepairing to make novel structures out of DNA. These structures can be used for DNA computing, but they don't have to be. Additionally, DNA computing can be done without using the types of molecules made possible by DNA Nanotechnology.
Nanoarchitecture
The idea of using DNA arrays to template the assembly of other functional molecules has been around for a while, but only recently has progress been made in reducing these kinds of schemes to practice. In 2006, researchers convalently attached
gold nanoparticles to a DX-based tile and showed that self-assembly of the DNA structures also assembled the nanoparticles hosted on them. The first report of assembly with a non-covalent hosting scheme came in 2007, using
Dervan polyamides on a DX array to arrange
streptavidin proteins on the DNA array.
Further Information
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